Coding

Part:BBa_K929104:Design

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-24)

Anti-GFP Nanobody


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus).


Source

Nanobody: anti-GFP nanobody taken from Protein Data Bank(PDB: 3OGO), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

References

Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401